Journal: Cell reports

Article Title: Human papillomavirus E5 suppresses immunity via inhibition of the immunoproteasome and STING pathway

doi: 10.1016/j.celrep.2023.112508

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-phospho-IRF3 (Ser396) (clone 4D4G) , Cell Signaling Technology , Cat#: 4947; RRID: AB_823547.

Techniques: Recombinant, cDNA Synthesis, SYBR Green Assay, Protease Inhibitor, Suspension, Activity Assay, Reporter Assay, Plasmid Preparation, Expressing, Software

Journal: bioRxiv

Article Title: 5,6-dimethylxanthenone-4-acetic acid (DMXAA), a Partial STING Agonist, Competes for Human STING Activation

doi: 10.1101/2023.12.07.570548

Figure Lengend Snippet: (A) Fresh human PBMCs from three healthy donors (n=3) were stimulated with the indicated CDNs (10 µg/ml) for 24 h after 90 minutes of DMXAA (100 µg/ml) pretreatment. IFNβ, IFNγ, and IL-6 production was measured using ELISA. Data from each individual from two independent experiments are shown as bar graphs. (B and C) PMA-differentiated THP1 dual reporter cells were pretreated with DMXAA (100 µg/ml) for 90 min and stimulated with the indicated CDNs (10 µg/ml) for 12 or 24 h in three independent experiments. After RNA isolation and cDNA synthesis, the mRNA expression levels of IFNβ (B) and IL-6 (C) were measured using RT-PCR. 18S ribosomal RNA was used as an internal control. IRF activity (B) was determined by measuring secreted luciferase activity in the supernatants. Representative data from three independent experiments are shown as the mean ± SD of triplicates (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, Student’s t test). (D) PMA-differentiated THP1 dual reporter cells were pretreated with DMXAA (100 µg/ml) for 90 min and stimulated with the indicated CDNs (10 µg/ml) for 3 h in three independent experiments. P-TBK1, TBK1, P-IRF3, and IRF3, levels in the cell lysates were detected using western blotting and the blots from a representative experiment were shown. Β-actin was used as loading control. Fold induction levels for P-TBK1 and P-IRF3 relative to total TBK1 and IRF3 from three independent experiments were plotted as mean ± SD (n=3) (*p < 0.05, **p < 0.01, Student’s t test).

Article Snippet: P-TBK1/NAK (Ser172, catalog # 5483), TBK-1/NAK (catalog # 3013), P-NF-Κb p65 (Ser536, catalog # 3033), NF-κB p65 (catalog # 8242), P-IRF3 (Ser396, catalog # 29047), IRF3 (catalog # 4302), and β-tubulin (catalog #86298) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Isolation, cDNA Synthesis, Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Activity Assay, Luciferase, Western Blot

Journal: PLoS ONE

Article Title: Recombinant Neuregulin 1 Does Not Activate Cardiomyocyte DNA Synthesis in Normal or Infarcted Adult Mice

doi: 10.1371/journal.pone.0115871

Figure Lengend Snippet: (A) Use of BrdU to monitor cardiomyocyte DNA synthesis in non-injured adult mice receiving 9 consecutive daily injections of NRG1β1 (BrdU was delivered using a mini-osmotic pump). Left panel shows anti-β-galactosidase immune reactivity, middle panel shows anti-BrdU immune reactivity, and right panel shows the merged image. Arrow indicates a BrdU positive cardiomyocyte nucleus, arrowhead indicates a BrdU positive non-cardiomyocyte nucleus. Bar = 10 microns. (B) BrdU incorporation in the nuclei of the small intestine microvilli epithelial cells of an NRG1β1-treated mouse. Note the absence of BrdU signal in the muscularis mucosae zone (asterisk). Bar = 10 microns. (C) Use of 3 H-Thy to monitor cardiomyocyte DNA synthesis in non-injured adult mice receiving 9 consecutive daily injections of NRG1β1 ( 3 H-Thy was delivered as a single bolus 1 hour after the last NRG1β1 treatment). Arrow indicates a 3 H-Thy positive cardiomyocyte nucleus, arrowhead indicates a 3 H-Thy positive non-cardiomyocyte nucleus. Bar = 10 microns.

Article Snippet: Experimental mice were treated with recombinant human NRG1β1 (corresponding to the EGF domain, amino acid residues 176–256, #396-HB, R&D Systems, Minneapolis, MN), at a dose of 2.5 micrograms per mouse per IP injection, dissolved in saline containing 0.1% Bovine Serum Albumin (BSA); control mice received vehicle alone.

Techniques: DNA Synthesis, BrdU Incorporation Assay

Journal: PLoS ONE

Article Title: Recombinant Neuregulin 1 Does Not Activate Cardiomyocyte DNA Synthesis in Normal or Infarcted Adult Mice

doi: 10.1371/journal.pone.0115871

Figure Lengend Snippet: Cardiomyocyte DNA synthesis in adult MHC-nLAC mice following vehicle or NRG1β1 injection.

Article Snippet: Experimental mice were treated with recombinant human NRG1β1 (corresponding to the EGF domain, amino acid residues 176–256, #396-HB, R&D Systems, Minneapolis, MN), at a dose of 2.5 micrograms per mouse per IP injection, dissolved in saline containing 0.1% Bovine Serum Albumin (BSA); control mice received vehicle alone.

Techniques: DNA Synthesis, Injection, Control

Journal: PLoS ONE

Article Title: Recombinant Neuregulin 1 Does Not Activate Cardiomyocyte DNA Synthesis in Normal or Infarcted Adult Mice

doi: 10.1371/journal.pone.0115871

Figure Lengend Snippet: (A) Western blot demonstrating the levels of total Erk1/2 p42/p44, P-Erk1/2[Thr 202 /Thy 204 ] and RLC in mice treated with NRG1β1 or vehicle (hearts harvested and processed 90 minutes after treatment). Densometric quantitation revealed that NRG1β1 treatment resulted in a 987% increase in the level of ERK1 phosphorylation, a 5727% increase in the level of ERK2 phosphorylation, and a 21% increase in the level of phosphorylated RLC vs. vehicle-treated mice (p<0.01, Student’s t-test). (B) Non-cardiomyocyte 3 H-Thy nuclear labeling index in non-injured adult mice following 9 consecutive daily injections of NRG1β1 (5 sections analyzed from each of 4 independent mice) or vehicle (4 sections analyzed from each of 4 independent mice). *: p<0.05 vs. vehicle treated animals, Student’s t-test.

Article Snippet: Experimental mice were treated with recombinant human NRG1β1 (corresponding to the EGF domain, amino acid residues 176–256, #396-HB, R&D Systems, Minneapolis, MN), at a dose of 2.5 micrograms per mouse per IP injection, dissolved in saline containing 0.1% Bovine Serum Albumin (BSA); control mice received vehicle alone.

Techniques: Western Blot, Quantitation Assay, Phospho-proteomics, Labeling

Journal: Foods

Article Title: Preparation, Biological Activities, and Potential Applications of Hen Egg-Derived Peptides: A Review

doi: 10.3390/foods13060885

Figure Lengend Snippet: Preparation methods of HEPs.

Article Snippet: , Chemical synthesis , Egg white protein , AAPPTEC 396 Automated Peptide Synthesizer. The 9-Fluorenylmethoxycarbonyloxy (Fmoc)-protected amino acid synthesis method peptide (RVPSLM). , α-glucosidase inhibitory activity , IC 50 23.07 μmol/L , [ ] .

Techniques: Membrane, Activity Assay, Recombinant, Foaming, Sonication, Control, De-Phosphorylation Assay, Antioxidant Activity Assay, Incubation, Concentration Assay, Emulsification

Journal: iScience

Article Title: Aberrant STING activation promotes macrophage senescence by suppressing autophagy in vascular aging from diabetes

doi: 10.1016/j.isci.2024.111594

Figure Lengend Snippet:

Article Snippet: Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb , Cell Signaling Technology , Cat#4947; RRID: AB_823547.

Techniques: Recombinant, Cell Isolation, Staining, ROS Assay, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, SYBR Green Assay, Plasmid Preparation, Software, Microscopy, Transmission Assay, Gene Expression